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. 2021 Jul 5;9(7):741. doi: 10.3390/vaccines9070741

Figure 3.

Figure 3

Titration of representative Influenza A (H1-H18) and influenza B (presplit, B/Victoria-like and B/Yamagata-like lineages) viruses. Pseudotyped lentiviral particles with HA envelopes: H1—A/England/195/2009(H1), H2—A/quail/Rhode Island/16-0186222-1/2016(H2), H3—A/ruddy turnstone/Delaware Bay/606/2017(H3), H4—A/green-winged teal/California/K218/2005(H4), H5—A/gyrfalcon/Washington/41088-6/2014(H5), H6—A/American wigeon/California/HS007A/2015(H6), H7—A/Shanghai/2/2013(H7), H8—A/mallard/Netherlands/7/2015(H8), H9—A/chicken/Israel/291417/2017(H9), H10—A/duck/Bangladesh/24268/2015(H10), H11—A/red shoveler/Chile/C14653/2016(H11), H12—A/northern shoveler/Nevada/D1516557/2015(H12), H13—A/laughing gull/New Jersey/UGAI7-2843/2017(H13), H14—A/mallard/Astrakhan/263/1982(H14), H15—A/duck/Bangladesh/24697/2015(H15), H16—A/black-headed gull/Netherlands/1/2016(H16), presplit—B/Hong Kong/8/1973, B/Victoria—B/Brisbane/60/2008, and B/Yamagata—B/Phuket/3073/2013, were titrated in HEK293T/17 cells. H17—A/little yellow-shouldered bat/Guatemala/60/2017(H17), and H18—A/flat-faced bat/Peru/33/2010(H18) were titrated in MDCKII cells. ΔHA is included as a no envelope control. Each point represents the mean and standard deviation of two replicates per dilution (n = 2). Readout is expressed in relative luminescence units (RLU).