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. 2021 Jul 13;9(7):782. doi: 10.3390/vaccines9070782

Figure 1.

Figure 1

Purification of rFu24 protein. (a) Purification of rFu24 by anion exchange chromatography using Q-Sepharose. Lanes 1–9: Ion exchange purified eluates of rFu24; (b) Purification of rFu24 by cation exchange chromatography using CMMC. Lanes 1–9: Mixed mode cation exchange purified eluates of rFu24; (c) rFu24 purity was analyzed by 15% SDS-PAGE gel under reducing and non-reducing conditions and stained with Coomassie blue. (d) Immunoblot of purified rFu24 protein detected with anti-PfMSPFu24 polyclonal mouse serum under reducing and non-reducing conditions. The arrow indicates rFu24. M, molecular weight marker; NR, non-reduced; R, reduced.