Figure 3.

Use of the MagnICON vector for expressing HBcAg-zDIII protein in N. benthamiana. The zDIII coding sequence was genetically fused to the 3′ end of the HBcAg gene (amino acid 1–155) and then cloned into the MagnICON-based plant expression vector pICH11599. This construct was transformed into Agrobacterium tumefaciens which was infiltrated into leaves of N. benthamiana to enable transient expression. At 7 days post-inoculation, the leaves were harvested to isolate the HBcAg-zDIII fusion protein. (Adapted from Yang et al., 2017 [14]).