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. 2021 Jul 2;9(7):723. doi: 10.3390/vaccines9070723

Figure 2.

Figure 2

Determination of desired antigen synthesis by Western blotting and immunofluorescent assay (IFA). The prokaryotic promoter PpagC synthesized HN proteins in MgM medium in pYL86 and pYL87 (A). M: prestained protein marker; 4545, pYL47, pYL86, and pYL87: Salmonella strain χ11218 harboring individual plasmids cultured in LB medium and MgM medium, respectively. The eukaryotic promoter CMV produced HN proteins that were confirmed by IFA (green), while the production of Cre recombinase was also confirmed (red)(B).