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. 2021 Jul 6;9(7):750. doi: 10.3390/vaccines9070750

Figure 2.

Figure 2

Stepwise stabilization leads to differences in biophysical protein traits. (a) nanoDSF and DLS analyses of all ConC-derived variants. Thermal transition curves (triplicates, mean in bold) are shown for one representative of three experiments. Intensity particle size distributions (10 acquisitions, mean in bold) are shown for one representative of three measurements. Melting temperatures (Tm) and particle sizes are indicated as mean ± SEM (standard error of mean) for each variant. ConC KIKO gp120: teal; ConCv1 KIKO NFL: orange; ConCv2 KIKO: lilac; ConCv4 KIKO: pink; ConCv5 KIKO: green. (b) Representative 2-D class averages of negative staining EM data of ConCv2 KIKO (left), ConCv4 KIKO (center), and ConCv5 KIKO (right). Indicated below each panel are the corresponding proportions of native vs. non-native trimers and closed vs. open trimers among native timers.