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Analytical sensitivity of the rRT-PCR assay. Serial ten-fold dilutions of quantitated in vitro-transcribed reference RNAs of different avian bornaviruses were used as templates in a singleplex assay with the respective probe ((A) BornaP_Fam; (B) BornaC_Aby; (C) BornaA_A647N) or in a multiplex assay where all three probes were used simultaneously (D–F). Results are presented as the mean CT values of two independent experiments.
