Table 3.
Impact of the presence of multiple avian bornavirus RNAs on the rRT-PCR performance. Comparison between multiple RNA targets vs. a single target RNA (106 copies per reaction).
| Probe Target | PaBV-1 | PaBV-2 | PaBV-4 | PaBV-7 | CnBV-1 | CnBV-2 | CnBV-3 | EsBV-1 | ABBV-1 | |
|---|---|---|---|---|---|---|---|---|---|---|
|
mean
CT |
st | 24.15 ±0.19 |
25.72 ±0.36 |
23.61 ±0.55 |
23.55 ±0.49 |
24.11 ±0.97 |
22.24 ±0.89 |
25.64 ±0.80 |
27.20 ±0.67 |
22.64 ±0.33 |
| mt | 24.04 ±0.15 |
29.36 ±0.34 |
25.26 ±0.18 |
21.99 ±0.20 |
28.19 ±0.68 |
23.52 ±0.84 |
25.49 ±0.91 |
24.45 ±0.87 |
23.72 ±1.03 |
|
| p-value | 0.5912 | 0.0002 | 0.0155 | 0.0170 | 0.0409 | 0.2299 | 0.8026 | 0.0130 | 0.1035 | |
Results are presented as average of four CT values +/− standard deviation. mt, multiple targets; st, single target. p < 0.05 was considered to indicated significant differences (analysed by Student’s t-test). st, single target; mt, multiple targets.