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Primer combination testing using 105 EID50 per RT-RPA reaction mixture (A). Analytical sensitivity of H9 RT-RPA using standard virus of titer 105 EID50/mL using a dilution range down to 1 EID50/mL (B). The combination H9 RPA-F1+ H9 RPA-R1+ H9 exo-probe was detected down to one RNA molecule. The discontinuity at 3 min 50 s due to the necessary mixing step interrupting fluorescence measurement.
