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Validation of the RT-RPA assay on the clinical samples compared to real-time RT-PCR. H9 RT-RPA threshold times (TT, Y-axis) and real-time RT-PCR cycle threshold values (CT, X-axis) were compared by PRISM software. R squared (R2) value was 0.12. Independent of the CT values, RT-RPA assay detected all samples within 7 min.
