Figure 1.

Study design. Twelve 4–5-month-old Holstein calves were divided into five groups for vaccination with GnGc, Gn only, or adjuvant only (placebo group). Calves were vaccinated subcutaneously at 0 and/or 21 days as indicated. At 35 days post first vaccination (dpv), calves were challenged with RVFV subcutaneously. Calf #15 died with acute RFV, and was necropsied at 3 days post challenge (dpc); the remaining calves were necropsied at 7 dpc and 10 dpc as indicated.