Figure 4.

RVFV plaque reduction neutralization assay of 80% (PRNT₈₀) determined a 0 and 7 days post challenge (dpc). Cattle were used to test the efficacy of a candidate RVFV subunit vaccines containing a combination of GnGc or Gn alone with or without a booster vaccination and the production of neutralizing antibodies was determined. Significance was determined by two-way ANOVA with Tukey’s multiple comparisons test for adjusted p values using Graph Pad Prism software. The PRNT₈₀ titers of GnGc/-, GnGc/GnGc and Gn/Gn groups were significantly different (* p value < 0.05) from placebo and -/Gn groups at 0 dpc, and all vaccinated groups were significantly different (* p value < 0.01) from the placebo group at 7 dpc.