Table 2.
Purification yield of target HTH1 proteins. Protein concentrations were measured spectrophotometrically estimating total amount of target recombinant protein in clarified lysate by combining densitometry calculation results and total soluble protein quantification results. CO—codon-optimized, CH—codon-harmonized, ND—target protein not detected in total soluble protein fraction.
| Identifier | Proposed Protein Function | Protein Purification Yield * (%) | |
|---|---|---|---|
| Target Protein Expressed from CO Gene Variant | Target Protein Expressed from CH Gene Variant | ||
| HTP4410 | N-acetylmuramoyl-L-alanine amidase | 85.6 ± 1.4 | 85.9 ± 1.9 |
| HTP4400 | rRNA biogenesis protein rrp5, putative | 38.6 ± 7.2 | 58 ± 3.7 |
| HTP4360 | hypothetical protein | 75 ± 3.8 | ND |
| HTP4350 | DUF262/DNase | 83.5 ± 5.2 | 92.1 ± 2.1 |
* Values represent mean ± standard error of three independent purifications.