Figure 2.

Protein expression of Mx1, ISG15, and CCL5 in TCRV-infected A549/Scr and A549/PKR cells. Cells were infected with TCRV at MOI of 0.01 PFU/cell and cell lysates were collected every 24 h. Total cellular proteins were probed for Mx1, ISG15 (A), and CCL5 (B), expression by Western blotting. Vinculin was included as a loading control. The ratios of Mx1, ISG15, and CCL5 versus Vinculin were calculated by densitometry at the corresponding day postinfection. One representative example out of three independent experiments is shown; (C) TCRV NP expression in A549/Scr and A549/PKR KO cells.