Table 1.
ISG profile of A549/Scr and A549/PKR KO cells infected with TCRV. Cells were infected at MOI of 0.01 PFU/cell and collected 5 days after infection. Values correspond to fold-induction (calculated by the 2−ΔΔCt method) and asterisks means statistical significance when compared to uninfected cells (* p < 0.01, ** p < 0.001) in two-way ANOVA test. For clarity, color intensity is proportional to up- or down-regulation (red and blue, respectively).
| ISG Name (Gene ID) | A549/Scr | A549/PKR KO | ISG Name | A549/Scr | A549/PKR KO |
|---|---|---|---|---|---|
| CCL5 (6352) | 12,673.81 ** | 5135.67 ** | RELA (5970) | 2.10 | 0.84 |
| IFNB1 (3456) | 5028.57 ** | 2020.63 ** | ATG5 (9474) | 1.87 | 1.61 |
| MX1 (4599) | 1046.76 ** | 1753.85 ** | IRF3 (3661) | 1.86 | 1.72 |
| ISG15 (9636) | 622.35 ** | 751.67 ** | APOBECC3G (60489) | 1.82 | 1.45 |
| OAS2 (4939) | 470.5 ** | 304.32 ** | TBK1 (29110) | 1.76 | 2.85 |
| IL6(3569) | 142.55 ** | 32.25 ** | RIPK1 (8737) | 1.74 | 0.81 |
| IFIH1 (64135) | 56.58 ** | 62.8 ** | CTSL (1514) | 1.72 | 2.27 |
| IRF7 (3665) | 35.41 ** | 14.54 * | IL15 (3600) | 1.71 | 1.26 |
| DDX58 (23586) | 30.11 ** | 38.58 ** | IL18 (3606) | 1.70 | 1.31 |
| CXCL8 (3576) | 12.4 ** | 7.49 ** | CD80 (941) | 1.56 | 2.82 |
| TLR3 (7098) | 10.65 ** | 12.05 ** | MAP2K1 (5604) | 1.54 | 1.99 |
| IL12A (3592) | 9.34 ** | 5.27 ** | CASP10 (843) | 1.52 | 0.89 |
| STAT1 (6772) | 9.25 ** | 8.39 ** | MAP3K7 (6885) | 1.49 | 1.48 |
| CYLD (1540) | 5.67 ** | 4.23 ** | DDX3X (1654) | 1.41 | 1.06 |
| TICAM1 (148022) | 3.7 ** | 1.23 | IRF5 (3663) | 1.41 | 1.10 |
| NFKB1A (4792) | 3.49 ** | 2.69 | IFNAR1 (15975) | 1.37 | 1.05 |
| IRAK1 (3654) | 3.23 | 0.77 | MAPK1 (5594) | 1.35 | 1.29 |
| TRIM25 (7706) | 3.22 ** | 1.64 | IKBKB (3551) | 1.30 | 0.64 |
| NFKB1 (4790) | 3.17 * | 1.40 | MAPK8 (5599) | 1.30 | 1.00 |
| MYD88(17874) | 3.11 ** | 2.86 ** | CASP8 (841) | 1.26 | 1.08 |
| TRADD (8717) | 2.94 | 2.53 | SPP1 (6696) | 1.21 | 2.07 |
| CD40 (958) | 2.8 | 1.45 | PIN1 (5300) | 1.16 | 1.53 |
| MAP2K3 (26397) | 2.78 ** | 1.96 ** | SUGT1 (10910) | 1.13 | 1.69 |
| AZI2(64343) | 2.73 * | 2.31 | HSP90AA1 (3320) | 1.05 | 1.27 |
| TRAF6 (7189) | 2.7 ** | 1.78 * | CARD9 (64170) | 1.04 | 0.78 |
| CHUK(1147) | 2.63 ** | 2.37 | MAPK14 (1432) | 0.97 | 0.67 |
| FADD (8772) | 2.63 * | 1.72 | MAPK3 (5595) | 0.93 | 0.45 |
| CTSS (1520) | 2.41 ** | 2.61 ** | CTSB (1508) | 0.91 | 1.30 |
| MAVS (57506) | 2.4 ** | 2.26 | IL1B (3553) | 0.85 | 0.83 |
| TRAF3 (7187) | 2.36 | 1.34 | PYCARD (29108) | 0.73 | 1.13 |
| CXCL9 (4283) | 2.13 | 3.21 | TKFC (26007) | 0.72 | 0.34 |
| MAP3K1 (4214) | 2.12 ** | 0.91 | FOS (14281) | 0.61 | 0.28 |
| JUN (3725) | 2.11 | 0.59 | IFNA1 (3439) | 0.61 | 0.37 |