Figure 8.

Clustered heatmap of the pairwise protein sequence similarity in endolysins. Every unique endolysin sequence in PhaLP was pairwise aligned against every other endolysin sequence to locate conserved subsequences in the pair. The resulting 4515 × 4515 alignment scores were subsequently normalized on length and clustered to find the most conserved subsequences in the database overall. This figure shows a heatmap of these alignment scores. The cluster discussed in the text is marked by the letter A. The complete set of clustered endolysins with annotation on architecture, accession and host is available in File S3.