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. 2021 Apr 7;138(3):259–272. doi: 10.1182/blood.2020006198

Figure 7.

Figure 7.

Plasminogen presence correlates with HK cleavage in situ. (A) Western blot of mouse liver homogenate after APAP exposure. Green = polyclonal antibody against the FXI- and PK-binding domain within the light chain of mouse HK. PLG knockout (−/−), heterozygous (+/−), and WT (+/+), and vehicle (saline) animals as indicated. Green arrow indicates full-length HK (∼120 kDa). Solid black arrow indicates plasmin-cleaved HK (∼60 kDa). Dashed black arrow indicates a cleavage product that increases with plasmin presence (∼52 kDa). (D) Plasma levels of ALT in PLG−/−, PLG+/−, PLG+/+, or saline vehicle mice 24 hours after APAP exposure. N = 6 mice per group. ***P < .001.