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. Author manuscript; available in PMC: 2021 Jul 24.
Published in final edited form as: J Vis Exp. 2020 May 12;(159):10.3791/61295. doi: 10.3791/61295

Figure 4. Reproducibility of Hoechst/PI staining.

Figure 4.

Viability of OCI-AML2 cells after 24 h of treatment with different concentrations of 3-bromopyruvate (3-BP). A. Total cell number counted via Hoechst 33342 staining, B. Dead cell number counted via propidium iodide staining. C. Viability (%) calculated using (A-B). D. Representative dose-response graph. Shown is mean with SEM. Three independent biological replicates were performed.