Fig. 4.

Bregs impair monocyte infiltration into the heart after MI. a Leukocytes were collected from the heart 1 and 3 day post-MI and stained for CD45, CD11b, Ly6G and Ly6C. Representative flow cytometric images of monocytes (gated on CD45⁺CD11b⁺Ly6G⁻) are shown. b–d Absolute numbers of monocytes and their subpopulations in the heart 1 day post-MI were analyzed. n = 8 per group. e–g Absolute numbers of monocytes and their subpopulations in the heart 3 day post-MI were analyzed. n = 5–6 per group. h Leukocytes were collected from the heart 7 day post-MI and stained for CD45, CD11b, Ly6G, F4/80 and CD206. Representative flow cytometric images of macrophages (gated on CD45⁺CD11b⁺Ly6G⁻F4/80⁺) are shown. i–k Absolute numbers of macrophages and their subpopulations in the heart at day 7 post-MI were analyzed. n = 5 per group. Data are expressed as means ± SEM. *P < 0.05, **P < 0.01. Data in b, c, g, i and k were analyzed by Kruskal–Wallis test with Dunn’s multiple comparisons test. Data in d–f and j were analyzed by one-way ANOVA, followed by Tukey’s post hoc test. PBS MI mice that received phosphate buffered saline, Breg MI mice that received regulatory B cells, Control B MI mice that received control B cells, Mo monocytes, MΦ macrophages