Fig. 5.

Breg transfer inhibits monocyte mobilization and recruitment. a–c Monocytes were collected from different tissues 1 and 3 days after MI and stained for CD45, CD11b, Ly6G and Ly6C. Representative flow cytometric images of monocytes are shown, respectively. d–f Absolute numbers of monocytes and their subpopulations in the spleen (d), bone marrow (e), and blood (f) at day 1 post-MI were analyzed. n = 8 per group. g–i Absolute numbers of monocytes and their subpopulations in the spleen (g), bone marrow (h), and blood (i) at day 3 post-MI were analyzed. n = 5–6 per group. Data are expressed as means ± SEM. *P < 0.05, **P < 0.01. Data in d, e and f were analyzed by one-way ANOVA, followed by Tukey’s post hoc test (monocytes and Ly6C^hi monocytes) or Kruskal–Wallis test with Dunn’s multiple comparisons test (Ly6C^lo monocytes). Data in g were analyzed by one-way ANOVA, followed by Tukey’s post hoc test (Ly6C^lo monocytes) or Kruskal–Wallis test with Dunn’s multiple comparisons test (monocytes and Ly6C^hi monocytes). Data in h and i were analyzed by one-way ANOVA, followed by Tukey’s post hoc test. PBS MI mice that received phosphate buffered saline, Breg MI mice that received regulatory B cells, Control B MI mice that received control B cells, Mo monocytes