Fig. 8.

Anti-IL-10 antibody antagonizes the effect of Bregs on monocytes in vivo. a Experimental procedures and timeline of surgery and treatment are shown. MI mice were administered with Bregs along with the anti-IL-10 antibody or isotype control antibody. b, c Numbers of monocytes and their subsets that infiltrated into the heart at day 1 (b) and day 3 (c) after MI were detected using flow cytometry. n = 6–7 per group. f CCR2 expression in monocytes from the spleen (d), bone marrow (e), and blood (f) was measured 1 and 3 day post-MI. n = 6–7 per group. Data are expressed as means ± SEM. *P < 0.05, **P < 0.01. Data in b were analyzed by one-way ANOVA, followed by Tukey’s post hoc test (monocytes and Ly6C^lo monocytes) or Kruskal–Wallis test with Dunn’s multiple comparisons test (Ly6C^hi monocytes). Data in c were analyzed by Kruskal–Wallis test with Dunn’s multiple comparisons test. Data in d were analyzed by one-way ANOVA, followed by Tukey’s post hoc test (3d) or Kruskal–Wallis test with Dunn’s multiple comparisons test (1d). Data in e and f were analyzed by one-way ANOVA, followed by Tukey’s post hoc test (1d) or Kruskal–Wallis test with Dunn’s multiple comparisons test (3d). MI MI mice control group, MI + Breg + Iso MI mice received regulatory B cells along with the isotype control antibody, MI + Breg + Anti-IL-10 MI mice received regulatory B cells along with the anti-IL-10 antibody, Ab antibody, Mo or mo monocytes, BM bone marrow