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. 2021 Jun;10(2):93–103. doi: 10.22099/mbrc.2021.39354.1578

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Extracellular matrix calcium deposits were visualized with Alizarin Red staining. A: control, B: BET and C: OD+BET groups. Magnification 100 .×D: Diagram of the amount of calcium phosphate through quantitative staining of Alizarin Red. Control (cultured cells in Osteogenesis Differentiation Medium); BET (cultured cells in α-MEM containing 10% FBS and Betaine); OD+BET (cultured cells in Osteogenesis Differentiation Medium containing Betaine). *Significant difference versus control, **Significant difference versus BET group