Figure 3.

Total soluble caspase-cleaved cytokeratin 18 (CCK18) released from (A) H727 and (B) H720 dying cells in the supernatants after treatment with AZ (40 μM), SFN (40 μM), and the combination AZ+SFN (40 μM each) for 72 hours using the caspase-cleaved cytokeratin 18 (M65 ^® , CCK18) CytoDeath ELISA Assay. The data are total CCK18 absorbance as a mean ± SEM of three independent experiments performed in triplicates. The results show a significant progressive increase in CCK-18 from AZ, SFN to the AZ+SFN combination. The expression of CCK18 was compared to the untreated control for each treatment group by ANOVA using the uncorrected Fisher’s LSD multiple comparisons test with 95% confidence with indicated asterisks for statistical significance. ^**** p ≤ 0.0001 n = 3. Abbreviations: AZ: acetazolamide; SFN: sulforaphane; CCK18: caspase-cleaved cytokeratin 18; ELISA: enzyme-linked immunosorbent assay; BC: bronchial carcinoma.