Figure 1. Experimental design for measuring CIN induced by telomere disfunction, based on the use of a linear EGFP-HAC versus a circular EGFP-HAC.

(A) Human fibrosarcoma HT1080 cells carried either the circular alphoid^tetO-HAC [38] or the linear 21DqHAC [37]. Cells that inherit a HAC display green fluorescence, while cells that lack a HAC do not. Both HACs are stable during cell division. Therefore, the control cells without drug treatment display uniform green fluorescence while in the drug-treated population there is a mixture of EGFP positive and negative cells. (B) The actual percentage of cells carrying an EGFP-HAC was measured by FACS as previously described [36].