Figure 2. Effect of terpyridine platinum compounds on a linear versus a circular EGFP-HAC mis-segregation rate.

(A) The chemical structures of Pt-tpy and its five derivatives Pt-cpym, Pt-vpym, Pt-ttpy, Pt(PA)-tpy, and Pt-BisQ; the terpyridine (tpy) core is shown in red and the aromatic surface expansion performed for each derivative is shown in blue. (B) HT1080 cells containing either a linear or a circular HAC were treated by Pt-tpy or its derivatives at concentrations corresponding to their LC₅₀ (Supplementary Table 1) overnight. The rate of HAC loss was calculated based on the ratio of HAC-positive to HAC-negative cells as described in Materials and Methods. The controls correspond to the frequency of spontaneous loss of the linear or circular HAC in HT1080 cells. The highest rate of the linear HAC loss was observed after treatment by Pt-cpym, Pt-vpym, Pt-ttpy, and Pt-tpy. ^*Indicates P-value < 0.05; ^** P < 0.01.