Fig. 3. The effect of 10 weeks standard chow diet (STD) or high fat diet (HFD) feeding on adipose tissue mitochondrial respiration in male and female mice.

Respiration stimulated with Complex I (glutamate and malate), Complex I ADP (ADP) Complex II (succinate) substrates, inhibition of UCP1 with GDP, maximally uncoupled electron transport chain (ETC) (FCCP) and respiratory control ratio (RCR) of permeabilized A iBAT, B iWAT and C eWAT of male and female mice fed either STD or HFD. Data are presented as mean ± SEM, Male STD (n = 10), Female STD (n = 9), Male HFD (n = 10), Female HFD (n = 10). Data are presented as mean ± SEM, differences were considered to be statistically significant at *P < 0.05, **P < 0.01.