Figure 3.

Secondary screening of top miRNA candidates targeting human PCSK9. (A) Relative luciferase activity of PCSK9 3′-UTR luciferase reporter construct in HEK-293T cells transfected with miRNA mimics for the top 35 hits identified from the primary screen. MiR-224 was included as a positive control. Data are presented as percent of control mimic ± standard deviation of three independent experiments. (B) Alignment of miRNA binding sites for the top 10 candidate miRNAs on the human PCSK9 3′-UTR. (C) qRT-PCR analysis of PCKS9 expression in HepG2 cells transfected with the top 10 candidate miRNAs or control mimic. Data are the mean ± standard deviation from three independent experiments. p-Values were calculated by one-way ANOVA and corrected for multiple comparison by Dunnet's post-hoc test (A) or a two-tailed Student's t-test (C). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.