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. 2021 Jul 12;8:667298. doi: 10.3389/fcvm.2021.667298

Figure 5.

Figure 5

PCSK9-targeting miRNAs regulate hepatic low-density lipoprotein receptor (LDLR) expression. (A) Western blotting analysis of LDLR and GAPDH protein in HepG2 cells transfected with miRNA mimics or a control mimic. Relative quantification of three independent experiments (mean ± standard error of the mean) is shown above the blots. (B) Representative fluorescent images of HepG2 cells stably expressing LDLR-GFP (green) transfected with miRNAs mimics or a control miR mimic. Counterstaining with DAPI to visualize nucleus (blue). (C,D) Quantification of (C) whole cell or (D) cell surface LDLR-GFP fluorescence intensity in HepG2 cells transfected with miRNA mimics or a control miR mimic. Data are representative of three independent experiments. p-Values were calculated using two-tailed Student's t-test. #p < 0.1, *p < 0.05, **p < 0.01, ***p < 0.001.