Figure 10.

Recovery of ⁷⁵Se from [⁷⁵Se]SeMet as ⁷⁵Se-labeled selenoproteins on SDS/PAGE gels. Rats were supplemented with 0.2 μg Se/g diet as selenite in the basal diet containing 0.4% supplemental Met for 50–80 d. For 7 days prior to Se injection, supplemental Met was adjusted to 0, 0.4, or 0.9% D,L-Met; rats were then injected iv with 50 μCi of [⁷⁵Se]SeMet,and tissues were subjected to SDS/PAGE as described for Figures 2–6 (n = 15 total rats). Values are the percent of the applied ⁷⁵Se recovered in the gel after SDS/PAGE in the indicated tissues at the indicated times.