TABLE 2.

Hematological analysis on day 5 in mitragynine replacement treatments.

Replacement groups	Vehicle	Mitragynine – Vehicle	Mitragynine 1 mg/kg Methadone	Mitragynine 0.8 mg/kg Buprenorphine	Mitragynine 1.1 mg/kg Clonidine	References range value
Total RBC (x 10∧12/L)	6.97 ± 0.24	8.23 ± 0.6	7.93 ± 0.35	8 ± 0.17	7.93 ± 0.37	6.39–8.01
Hemoglobin (gm/L)	155.67 ± 8.19	168 ± 12.7	168 ± 4.36	159 ± 8.02	156.67 ± 9.61	135–159
PCV (%)	49.33 ± 1.86	47 ± 0.04	45 ± 1.73	46.33 ± 1.76	43.67 ± 0.02	42–49
MCV (fL)	58.33 ± 2.03	57 ± 1.73	58 ± 0.58	59 ± 2.08	55 ± 1.15	58.01–67.00
MCH (pg)	20.67 ± 0.33	20.33 ± 0.33	21 ± 0.58	19 ± 1.53	19.67 ± 0.33	18.70–21.20
MCHC (g/L)	316.67 ± 8.82	360 ± 5.77	376.67 ± 8.82*	320 ± 20	360 ± 5.77	310–336
RDW (%)	15.17 ± 0.42	14.93 ± 0.94	14.93 ± 0.12	15.4 ± 0.58	14.93 ± 0.26	13.03–16.57
Total WBC (x 10^9/L)	4.2 ± 1.18	4.8 ± 1.29	4.4 ± 0.71	6.47 ± 0.18	5.13 ± 0.96	3.00–9.22
Lymphocytes (%)	76.33 ± 2.03	66 ± 5.86	65.67 ± 6.64	76.33 ± 1.45	69 ± 1.53	51.8–89.7
Monocytes (%)	2.67 ± 0.67	0.33 ± 0.33	3 ± 0.58	2.67 ± 0.67	0.67 ± 0.33	1.3–6.0
Eosinophils (%)	0 ± 0	0.33 ± 0.33	0.33 ± 0.33	0.58 ± 0.01	0 ± 0	0.5–7.2
Basophils (%)	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0 ± 0	0–0.6
Platelet count (x 10∧9/L)	682 ± 112.65	529 ± 62.69	702.33 ± 18.89	946.33 ± 3.53#	483.33 ± 39.54	529.0–1,383.0

Total RBC, red blood cell count; PCV%, percentage of packed cell volume; MCV, mean corpuscular volume; MCH, mean corpuscular haemoglobin; MCHC, mean corpuscular haemoglobin concentration; RDW%, percentage of red cell distribution width; WBC, total of white blood cell count, Lymphocytes (%), Percentage of lymphocyte; Monocytes (%), Percentage of monocytes; Eosinophils (%), Percentage of eosinophils; Basophils (%), Percentage of basophils; PLT, Platelet counts.

Data represent means ± SEM of n = 6 rats/group *p < 0.05 vs. Vehicle, #p < 0.05 vs. Mitragynine-Vehicle, analysed by one-way repeated measures ANOVA and Bonferroni post-hoc test.