Figure 2.

Overexpression of HOXD8 inhibits the proliferation, migration and invasion of MCF-7 breast cancer cells. (A) Transfection efficiency of pcDNA-HOXD8 was determined using western blotting. Cell proliferation was detected using (B) Cell Counting Kit-8 and (C and D) colony formation assays following overexpression of HOXD8. (E and F) Cell migration was analyzed using a wound healing assay. (G and H) Cell invasion was assessed using a Matrigel Transwell assay. (I) MMP2 and MMP9 protein expression was analyzed using western blotting. ^**P<0.01 and ^***P<0.001 vs. pcDNA-NC; n≥3. Scale bar, 100 µm. HOXD8, homeobox D8; NC, negative control.