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. 2021 Jul 15;22(3):1006. doi: 10.3892/etm.2021.10439

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Copyright: © Wen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Knockdown of ILP2 reverses the promoting effects of HOXD8 knockdown on the proliferation, migration and invasion of breast cancer cells. (A) Transfection efficiency of shRNA-ILP2 was determined using western blotting. Cell proliferation following ILP2 knockdown was analyzed using (B) Cell Counting Kit-8 and (C and D) colony formation assays. (E and F) Cell migration was measured using a wound healing assay. (G and H) Cell invasion was determined using a Matrigel Transwell assay. (I) MMP2 and MMP9 protein expression was analyzed using western blotting. ^*P<0.05, ^**P<0.01, ^***P<0.001 vs. control; ^#P<0.05, ^###P<0.001 vs. shRNA-HOXD8 + shRNA-NC; n≥3. Scale bar, 100 µm. HOXD8, homeobox D8; ILP2, inhibitor of apoptosis-like protein-2; NC, negative control; shRNA, short hairpin RNA.