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. 2021 Jul 12;9:700029. doi: 10.3389/fcell.2021.700029

FIGURE 2.

FIGURE 2

Effects of DGCR5 isoform-1 on RCC cell proliferation and cell cycle. (A) DGCR5 mRNA expression detected by qPCR in A704 cells. Cells were transfected with si664- or si523-targeting DGCR5 or siRNA-NC for 48 h. The mRNA expression of DGCR5 was normalized to GAPDH. Effects of DGCR5 knockdown on cell viability, cell proliferation, and cell-cycle regulation, which is detected by CCK8 assay (B), EdU assay (C, magnification ×100), and cell flow cytometry analysis (D), respectively. All experiments were performed in triplicate and repeated in A704 cells. Data are shown as mean ± SD; ##P < 0.0001. siNC, small interfering RNA negative control; si664, small interfering RNA 664 targeting DGCR5; si523, small interfering RNA 523 targeting DGCR5; CCK8, cell counting kit-8; OD, optical density; EdU, 5-ethynyl-2′-deoxyuridine.