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. 2021 Jul 12;9:700029. doi: 10.3389/fcell.2021.700029

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Copyright © 2021 Zhong, Luo, Fan, Wang, Liu, Xu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DGCR5 isoform-1 targets miR-211-5p in vitro. (A) The flow diagram of screening and validation of potential miRNA targets of DGCR5. (B) The luciferase reporter plasmids were constructed containing WT or MUT MREs for miR-211-5p of the DGCR5 sequence. (C) The luciferase reporter assay was analyzed in HEK293 cells cotransfected with plasmids containing WT or MUT MREs of DGCR5 sequence and miR-211-5p mimics. Independent experiments were carried out in triplicate. Data are shown as mean ± SD; ^#P < 0.001. miR, microRNA; WT, wild type.