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. 2021 Jul 12;9:700029. doi: 10.3389/fcell.2021.700029

FIGURE 7.

FIGURE 7

The effects of DGCR5 isoform-1 on RCC cell Snail expression and its downstream gene transcription by targeting miR-211-5p. Western blot was performed to detect Snail protein expression (A,B), and qPCR was performed to detect E-cadherin and CLDN7 mRNA expression (C,D) in A704 cells treated with siRNA targeting DGCR5 (si664 or si523), miR-211-5p inhibitors or both siRNA and miR-211-5p inhibitors. All experiments were performed in triplicate. Data are shown as mean ± SD; #P < 0.001 and ##P < 0.0001.