Figure 9.

PE6 inhibits the formation of LC3BII punctate foci in treated RAW264.7 macrophage cells in the presence of pharmacological agents rapamycin and bafilomycin A1. (A) Analysis of immunofluorescence microscopic images showing the LC3BII foci in untreated, rapamycin (200 nM) treated, HI + rapamycin-treated, and PE6 + rapamycin-treated RAW264.7 cells at 24 and 48 h. DAPI was used to mark the nucleus. Untreated and rapamycin-treated cells were used as negative and positive controls, respectively. (B) Immunofluorescence microscopic images demonstrating the LC3BII foci in untreated, HI treated, and PE6 treated RAW264.7 cells in the presence of bafilomycin A1 (50 nM). Untreated and bafilomycin A1 treated cells were used as negative and positive controls. (C, D) The average number of LC3BII punctate foci were counted using Axio-vision software and represented as a bar graph. Scale bars indicate 10 µM. Data were analyzed using paired T-test with the Kruskal–Wallis post-test. Data are representative of three independent experiments. ****P < 0.001 vs. controls.