Figure 5.

Specificity of sensors and their compatibility in serum. (A) Specificity test of the sensor using 100 pM target/mutants. While the target is perfectly complementary, mutants have one or two mismatched nucleotides (bolded and underlined). We found that 70% of molecules were dynamic in the presence of 100 pM target, while a negligible fraction of molecules showed dynamic behavior in the presence of 100 pM mutant, thus demonstrating a high specificity of our approach. (B) Sensors behave similarly in 1× Tris HCl and 10% human serum. Notice the zero background in the absence of the target.