Figure 5.

Upregulation of MSI2 could reverse the role of si-SOX2-OT in HCC. pcDNA3.1-MSI2 was delivered into HepG2 cells which were transfected with si-SOX2-OT#2, and the cells with si-NC + pcDNA3.1-NC were used as control. Then, (A) RT-qPCR was used to verify the intervention effect of pcDNA3.1-MSI2; (B) CCK-8, (C) colony formation assay, and (D) Transwell assays were utilized to assess proliferation, invasion, and migration of the cell; (E) After 4 weeks of subcutaneous tumorigenesis in nude mice, tumor bodies were obtained and tumor weight was measured; (F) Changes of tumor volume within 4 weeks after subcutaneous tumorigenesis in nude mice and typical pictures; (G) The levels of SOX2-OT, miR-143-3p and MSI2 protein in tumor bodies were detected by RT-qPCR. There were 6 mice in each group. The cell experiment was repeated three times independently and data were all metrological data. All data were shown as mean ± standard deviation. One-way ANOVA was used for data analysis, followed by Tukey’s test. *P < 0.05, **P < 0.01, ***P < 0.001.