Table 4. Hospitalised COVID-19 positive patient stool sample results using the Viasure Real-Time PCR SARS-CoV-2 detection kit.
| Viasure validated data | SARS-CoV-2 Detected | RT-ddPCR
Concentration (copy number/µl purified RNA) |
|||
|---|---|---|---|---|---|
| Patient Number | Ct value
ORF1ab gene |
Ct value
N gene |
Ct value
IC |
||
| P01 | UD | UD | 24.69 | N | 0 |
| P02 | UD | UD | 23.23 | N | 0 |
| P03 | 28.02 | 24.33 | 24.59 | Y | 10.7 |
| P04 | UD | UD | 24.26 | N | 0 |
| P05 | 37.21 | 23.60 | 23.86 | Y | 0 |
| P06 | UD | UD | 23.89 | N | 0 |
| P07 | UD | UD | 23.81 | N | 0.8 |
| P08 | 32.75 | 23.24 | 24.04 | Y | 1 |
| P09 | 28.34 | 23.68 | 24.26 | Y | 8.9 |
| P10 | UD | UD | 24.04 | N | 0 |
| P11 | 34.10 | 23.09 | 23.43 | Y | 0.8 |
| P12 | UD | UD | 23.78 | N | 0 |
| Extraction Positive control | 20.98 | 19.91 | 23.56 | Y | 1278 |
| Extraction negative control | UD | UD | 23.43 | N | 0 |
| Viasure PCR positive control | 20.46 | 19.72 | 24.32 | Y | 295* |
| PCR negative control | UD | UD | 24.10 | N | 0 |
UD: Undetermined- Below lower detection limits
• E gene positive control 10 -3 ATCC control