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. 2021 May 11;10:373. [Version 1] doi: 10.12688/f1000research.52178.1

Table 4. Hospitalised COVID-19 positive patient stool sample results using the Viasure Real-Time PCR SARS-CoV-2 detection kit.

Viasure validated data SARS-CoV-2 Detected RT-ddPCR
Concentration
(copy number/µl
purified RNA)
Patient Number Ct value
ORF1ab gene
Ct value
N gene
Ct value
IC
P01 UD UD 24.69 N 0
P02 UD UD 23.23 N 0
P03 28.02 24.33 24.59 Y 10.7
P04 UD UD 24.26 N 0
P05 37.21 23.60 23.86 Y 0
P06 UD UD 23.89 N 0
P07 UD UD 23.81 N 0.8
P08 32.75 23.24 24.04 Y 1
P09 28.34 23.68 24.26 Y 8.9
P10 UD UD 24.04 N 0
P11 34.10 23.09 23.43 Y 0.8
P12 UD UD 23.78 N 0
Extraction Positive control 20.98 19.91 23.56 Y 1278
Extraction negative control UD UD 23.43 N 0
Viasure PCR positive control 20.46 19.72 24.32 Y 295*
PCR negative control UD UD 24.10 N 0

UD: Undetermined- Below lower detection limits

•   E gene positive control 10 -3 ATCC control