Table 2.

Kinetic parameters of phenolic acid substrates of P-WT and mutants

		Kinetic constanta
		P-Coumaric acid	Ferulic acid	Caffeic acid	Sinapic acid
P-C	Vmax (IU/mg)	193.80 ± 2.38	97.73 ± 5.86	65.66 ± 4.79	12.36 ± 0.79
	Km (mmol/L)	2.05 ± 0.29	1.03 ± 0.38	3.04 ± 0.69	1.31 ± 0.03
	kcat/Km (mmol/L/s)	13.41 ± 0.45	28.15 ± 0.28	23.24 ± 0.52	40.87 ± 0.28
P-N	Vmax (IU/mg)	683.10 ± 7.38	208.50 ± 8.38	143.80 ± 5.89	10.36 ± 0.34
	Km (mmol/L)	7.02 ± 0.61	2.14 ± 0.83	1.48 ± 0.73	1.06 ± 0.42
	kcat/Km (mmol/L/s)	14.53 ± 0.53	33.57 ± 0.49	37.57 ± 0.62	28.44 ± 0.53
P-m1	Vmax (IU/mg)	17.26 ± 0.38	19.07 ± 8.39	124.60 ± 3.86	16.66 ± 0.78
	Km (mmol/L)	1.88 ± 0.53	2.08 ± 0.28	1.36 ± 0.28	1.82 ± 0.38
	Kcat/Km (mmol/L/s)	152.38 ± 1.12	28.75 ± 0.39	32.14 ± 0.52	19.17 ± 0.19
P-m2	Vmax (IU/mg)	136.20 ± 8.36	111.90 ± 10.91	72.64 ± 6.89	5.08 ± 0.58
	Km (mmol/L)	1.22 ± 0.26	1.00 ± 0.38	6.49 ± 0.53	4.55 ± 0.61
	kcat/Km (mmol/L/s)	15.97 ± 0.27	36.54 ± 0.36	4.07 ± 0.64	13.24 ± 0.66
P-Nm1	Vmax (IU/mg)	254.40 ± 7.38	103.20 ± 7.48	87.37 ± 8.64	5.60 ± 0.53
	Km (mmol/L)	3.52 ± 0.32	1.43 ± 0.06	1.21 ± 0.43	0.77 ± 0.64
	kcat/Km (mmol/L/s)	11.31 ± 0.36	19.94 ± 0.73	24.72 ± 0.63	45.67 ± 0.25
P-Nm2	Vmax (IU/mg)	186.30 ± 6.39	99.09 ± 0.3.83	144.20 ± 7.32	5.27 ± 0.17
	Km (mmol/L)	12.05 ± 0.27	6.4 ± 0.38	9.33 ± 0.28	3.41 ± 0.26
	kcat/Km (mmol/L/s)	10.33 ± 0.18	14.14 ± 0.59	10.38 ± 0.97	11.69 ± 0.11
P-WT	Vmax (IU/mg)	121.50 ± 7.88	22.32 ± 4.31	89.46 ± 10.35	9.22 ± 0.19
	Km (mmol/L)	4.09 ± 0.59	2.39 ± 0.58	3.10 ± 0.47	3.20 ± 0.30
	kcat/Km (mmol/L/s)	9.74 ± 0.53	16.49 ± 0.42	9.76 ± 0.26	16.94 ± 0.58

^a These values were calculated by taking the average of three experiments conducted using SD