Skip to main content
. 2021 Jul 12;12:675294. doi: 10.3389/fimmu.2021.675294

Figure 5.

Figure 5

IL-1 receptor signaling reduces neutrophil apoptosis in vitro. (A) Neutrophil cell death as measured by LDH release following co-culture of bone-marrow isolated neutrophils from the indicated mouse strains with sterile agar beads (Sham) or A. fumigatus conidia-containing beads (AfB) for 24 hours. Results represent mean ± SD of 2 independent experiments, ***p ≤ 0.001, (one-way ANOVA test). (B) Neutrophil cell death as measured by 7-AAD staining and flow cytometry analysis of bone-marrow isolated neutrophils from the indicated mouse strains directly after isolation (0 Hour) or after 24 hours of culture in 10% FBS supplemented RPMI. Results represent means ± SD of 3 independent experiments, **p ≤ 0.01, (one-way ANOVA test). (C) Apoptosis of bone-marrow isolated neutrophils from the indicated mouse strains as measured by Annexin V staining either directly after isolation (0 hour) or after 24 hours of culture. Results represent mean ± SD of 3 independent experiments, **p ≤ 0.01, (one-way ANOVA test).