Table 2.
Specific NADH dehydrogenase activity with and without inhibitors
| Species | Specific activity (U mg−1) | Inhibitor | Inhibition (%) |
|---|---|---|---|
| Phytomonas serpens | 28 ± 11 | Rotenone | 30 ± 4 |
| Capsaicin | 42 ± 6 | ||
| DPI | 37 ± 4 | ||
| Kentomonas sorsogonicus | 39 ± 8 | Rotenone | 2 ± 3 |
| Capsaicin | 9 ± 8 | ||
| DPI | 100 ± 0 | ||
| N. esmeraldas | 20 ± 9 | Rotenone | 9 ± 7 |
| Capsaicin | 34 ± 12 | ||
| DPI | 35 ± 8 | ||
| Sergeia podlipaevi | 27 ± 10 | Rotenone | 6 ± 3 |
| Capsaicin | 27 ± 9 | ||
| DPI | 20 ± 4 | ||
| W. raviniae | 110 ± 24 | Rotenone | 7 ± 4 |
| Capsaicin | 8 ± 2 | ||
| DPI | 81 ± 13 |
NADH dehydrogenase activity was measured in the mitochondrial lysates of P. serpens, K. sorsogonicus, N. esmeraldas, S. podlipaevi and W. raviniae in the absence or presence of 10 μm rotenone, 300 μm capsaicin and 100 μm DPI. Average values and s.d. of activities and their inhibition (in %) from at least three independent biological replicated (each measured in triplicates) are presented. One unit (U) of NADH dehydrogenase activity catalyses the oxidation of 1 nmol NADH per minute. Specific activity is calculated as U mg−1 of mitochondrial proteins.