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. 2021 Jul 12;12:695155. doi: 10.3389/fmicb.2021.695155

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Copyright © 2021 Fu, Tan, Shi and Xun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Positive model (A,C) and negative model (B,D). PLS-DA scores: The abscissa represents the first principal component PC1, and the ordinate represents the second principal component PC2. Replacement test diagram: R2 stands for model verification, and the Y matrix of original classification and N times of different arrangement are linearly regressed with R2Y and Q2Y, and the intercept values of regression line and y-axis are R2 and Q2, respectively; used to measure whether the model is over-fitted.