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. 2021 Jul 21;16:4913–4928. doi: 10.2147/IJN.S317113

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© 2021 Zhao et al.

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Physicochemical properties of Ng(-)pIL-12. (A) The preparation of Ng(-)pIL-12 chitosan nanoparticle. (B) Transmission electron microscopy (TEM) images of Ng(-)pIL-12. Scale bar represents 200 nm. (C) Particle size and Zeta potential of Ng(-)pIL-12. (D) Gel retardation analysis of Ng(-)pIL-12 at different nanogel/pIL-12 mass ratios as 4:1, 8:1, 16:1, respectively. (E) In vitro toxicity of Ng(-)pIL-12 and naked pIL-12 at different indicated concentrations in BMDCs and splenocytes, and the O.D. of PBS-treated group (PBS) was identified as 1. pIL-12, naked pIL-12; Ng(-)pIL-12, Ng(-) containing pIL-12. One representative from three independent experiments.