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. 2021 Jul 23;9:e11817. doi: 10.7717/peerj.11817

Figure 1. High glucose upregulated NEAT1 and SOX4, and downregulated miR-204.

Figure 1

(A) qRT-PCR analysis of NEAT1, SOX4 and miR-204 in ARPE19 cells at 12, 24, 48 and 72 h after high glucose induction. (B–C) SOX4 and EMT-associated proteins such as E-cadherin, N-cadherin, Snail, and Vimentin were examined by western blot (B) and cell immunofluorescence (C) at 12, 24, 48 and 72 h after high glucose induction, Scale bar: 50 µM. D. Cell migration was investigated by wound healing assay, the first row was the scratch size of control and high glucose treatment (12, 24, 48 and 72 h) group at 0 h, which was used as a baseline comparison, while the second row was the scratch size of corresponding groups at 72 h, which could be used to evaluate the treatment effects against high glucose by comparing with the first row. E. invasion ability was investigated by transwell assay E at 12, 24, 48 and 72 h after high glucose induction. Data were presented as the mean ± SD of three separated experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.