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. 2021 Jul 23;9:e11817. doi: 10.7717/peerj.11817

Figure 2. Knockdown of NEAT1 inhibited high glucose-induced EMT.

Figure 2

(A) ARPE19 cells was first transfected with si-NEAT1, then the expression level of NEAT1, SOX4 and miR-204 after 72 h high glucose induction were analyzed by qRT-PCR. (B) Cell viability were verified by MTT assay in experiments described in A. (C–D). SOX4 and EMT-associated proteins such as E-cadherin, N-cadherin, Snail, and Vimentin were examined by western blot (C) and cell immunofluorescence (D) after 72 h high glucose induction and 72 h si-NEAT1 transfection. Scale bar: 50 µM. (E–F). Cell migration and invasion ability was investigated by wound healing assay (E) and cell transwell assay (F). Data were presented as the mean ± SD of three separated experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.