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. 2021 Jul 23;9:e11817. doi: 10.7717/peerj.11817

Figure 5. NEAT1 could function as a ceRNA to affect SOX4 through sponging miR-204.

Figure 5

(A) SOX4 in ARPE19 cells after 72 h high glucose induction and 72 h si-SOX4 transfection was analyzed by qRT-PCR. (B–C). Cell viability and cell proliferation were verified by MTT assay(B) and BrdU assay (C) respectively after 72 h high glucose induction and 72 h si-SOX4 transfection. (D–E). SOX4 and EMT-associated proteins such as E-cadherin, N-cadherin, Snail, and Vimentin were examined by western blot (D) and cell immunofluorescence (E) after 72 h high glucose induction and 72 h si-SOX4 transfection. Scale bar: 50 µM. (F–G) Cell migration and invasion ability was investigated by wound healing assay (F) and cell transwell assay (G) after 72 h high glucose induction and 72 h si-SOX4 transfection. Data were presented as the mean ± SD of three separated experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.