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. 2021 Jul 23;9:e11817. doi: 10.7717/peerj.11817

Figure 6. NEAT1 regulated high glucose-induced EMT through miR-204/SOX4 pathway.

Figure 6

(A) SOX4 in ARPE19 cells after 72 h high glucose induction and 72 h si-NEAT1 and miR-204 inhibitor co-transfection was analyzed by qRT-PCR. (B–C). Cell viability and cell proliferation were verified by MTT assay (B) and BrdU assay (C) respectively after 72 h high glucose induction and 72 h si-NEAT1 and miR-204 inhibitor co-transfection. (D) SOX4 and EMT-associated proteins such as E-cadherin, N-cadherin, Snail, and Vimentin were examined by western blot after 72 h high glucose induction and 72 h si-NEAT1 and miR-204 inhibitor co-transfection. (E–F) Cell migration and invasion ability was investigated by wound healing assay and cell transwell assay after 72 h high glucose induction and 72 h si-NEAT1 and miR-204 inhibitor co-transfection. Data were presented as the mean ± SD of three separated experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.