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. 2021 Jul 12;12:676671. doi: 10.3389/fmicb.2021.676671

TABLE 1.

Experimental designs with mycotoxin alone or synergetic inoculations.

Groups Toxins Doses (μg/egg)
1 FB1 24
12
6
2 FB2 48
24
12
3 FB1 + FB2 + FB3 24 (14.4 + 4.8 + 4.8)
12 (7.2 + 2.4 + 2.4)
6 (3.6 + 1.2 + 1.2)
4 FB1 + DON 0.1 + 12
0.1 + 6
0.1 + 3
5 DON 0.1
6 CONTROL NS

All embryonated eggs were randomly divided into six groups, namely, four experimental groups (each experimental group consisting of the high-dose group, the moderate-dose group, and the low-dose group, eight replicates per group) and two control groups (the DON control group and the methanol group, eight chicken embryos per group). Before inoculation, poorly growing embryonated eggs were eliminated from the experiment.