Figure 1.

Culture protocol to obtain phenotypically and functionally mature T cells from CD34⁺ HSPC. Numbers in bold indicate time (in days) of in vitro culture. Red arrows (bottom) indicate points of addition of different cytokine mixes, i.e. cytokine mix 1 during agonist selection and cytokine mix 2 during polyclonal feeder expansion. Blue arrows (top) indicate read-out timepoints, i.e. after agonist selection (read-out 1) and after polyclonal feeder expansion (read-out 2). Abbreviations: CB, cord blood; SCF, stem cell factor; FLT3-L, FMS-like tyrosine kinase 3 ligand; IL-7, interleukin-7; DP, double positive; RV, retroviral