Figure 4.

Addition of IL-21 during maturation induces mature T cells with a less-differentiated phenotype. Phenotype of cells after agonist selection, gated on eGFP⁺ CD1a⁻ transduced mature cells, or eGFP⁺ CD1a⁻ CD62L⁺ cells for (c). Gating strategy is shown in Fig S6. Cells were agonist selected with the cognate peptide in the presence of cytokine(s) as shown. Percentage of CD45RA⁺ CD62L⁺ (a), CD62L⁺ (b), NKG2D⁺ (d), CD95⁺ (f) cells. (c) Mean fluorescence intensity (MFI) for CD62L. For (a–d and f) individual values and mean ± SD of 5 donors is shown. Results were compared using Kruskal–Wallis test followed by Dunn’s multiple comparisons test. p-value < 0.05 (*), p-value < 0.01 (**), p indicates p-value. (e) Expression (left) and MFI (right) for CD95 for one representative in vitro culture (of a total of 5 donors). CD95 expression and MFI for the other 4 donors are shown in Supplementary Figure S8